Fig. 1. Identification of novel phage endolysin ST01.
(A) Cladogram of ST01 with 18 proteins using the Constraint-based Multiple Alignment Tool (COBALT). (B) Multiple alignment analysis of amino acid sequence of ST01 with Salmonella phage vB_SenS_ER21 lysozyme, Salmonella virus VSe101 lysin, and Salmonella phage vB_SenS_ER22 lysozyme. The alignment was performed using ClustalW. Black boxes represent 100% sequence identity. (C) The domain structure of ST01 was predicted by the InterPro tool. The predicted lysozyme-like domain is located between amino acid 34 and 140 of ST01.