Fig. 2. Pairwise alignment (A) and phylogenetic relationship (B) of Burkholderia pseudomallei, Bacillus subtilis, Escherichia coliO157, Escherichia coliK-12, Pseudomonas aeruginosa, and Schizosaccharomyces pombe L-asparaginase.
Red asterisks show the conserved segment near the N-terminal end and the blue asterisks show the conserved threonine residues representing the catalytic triad threonine 113, 117, 124, 222 involved in catalysis (A). Maximum probability tree is based on GenBank-deposited full coding sequences (B).