JMB

eISSN 1738-8872
pISSN 1017-7825

Table. 4.

Table. 4.

Applications of violacein in synthetic biology.

Applications Reference
Reporters Improvement of the biocatalytic efficiency of vioABE having prodeoxyviolacein (PDV) or its derivative, with visible green and red fluorescence. Estimation of the permeability of peroxisomal membrane (up to the molecular weight between 571-733 Da) by the fusion of optimized tag ePTS1 to β-glucosidase, VioA, B, or E. [26]
Benzylisoquinoline alkaloids (BIAs) Application of a well-characterized translocation system for the catalysis of the first step for the biosynthesis of BIAs from Coptis japonica in S. cerevisae to sequester tNCS.
Induction of larger peroxisomes by addition of oleate to improve BIA production.
[113]
T7 Promoter mutant library VioA, B, C, D, and E libraries were built up using high-strength promoters through which the violacein production can be directly evaluated. Such fine-tuning method for multiple genes called ePathOptimize balances the metabolic flux. [119]
Golden Gate assembly Evaluation of the efficiency of gene assembly toolkits developed via Golden Gate assembly in E. coli and yeast. [114, 115]
Biosensors The construction of a highly sensitive whole-cell biosensor that can detect Pb (II) concentration as low as 0.1875 μmol, was designed under the control of T7 lac promoter in E. coli. [27]
Hg(II) biosensor controlled by mer promoter and MerR regulator to assess the ecotoxicity of environmental water samples having mercury pollutants [124]
Combinatorial libraries Combinatorial libraries for matching promoters with the adjacent suitable genes and characterization of constitutive promoters for maximum titer production of violacein. [25]
Artificial Protein Scaffolds (AProSS) VioC, VioD, and VioE were brought in proximity via Artificial Protein Scaffolds (AProSS) increased the yield of violacein and deoxyviolacein by 29% and 63% respectively. [117]
Bistable switch Application of a Bistable switch in Saccharomyces cerevisiae for controlling vioC and vioD which switches on alternative vioC and vioD pathways on demand. [118]
YeastFab Assembly strategy (MiYA) MiYA was developed in S. cerevisiae and could allow the identification of a combination with 2.42-fold improved violacein production among 3125 possible designs and predict the synthesis of pure violacein, avoiding the branch pathway. [116]
J. Microbiol. Biotechnol. 2021;31:1465~1480 https://doi.org/10.4014/jmb.2107.07045
© J. Microbiol. Biotechnol.