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Fig. 3.

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Fig. 3. Apoptotic effects of MRBE on RPMI-8226 cell growth. (A) Cytometry analysis of RPMI-8226 cells incubated with either DMSO (vehicle) or MRBE (20 and 40 μg/ml) for 48 h. After incubation, cells were harvested and stained with annexin V-FITC and propidium iodide (PI). The x-axis indicates the annexin V-FITC intensity, while the y-axis indicates the PI fluorescence. (B) Western blot with anti-caspase-3, anti-poly (ADP-ribose) polymerase (PARP), and anti-actin (loading control) antibodies on whole-cell extracts. The results are representative of three independent experiments. (C) Caspase-3/7 activity determined after RPMI8226 cell incubation with either DMSO or MRBE (20 and 40 μg/ml) for 48 h. The results represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 compared with the DMSO control group.
J. Microbiol. Biotechnol. 2021;31:1559~1567 https://doi.org/10.4014/jmb.2109.09002
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