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Table. 2.

Table. 2.

Characteristics of open reading frames (ORFs) in the O-antigen gene clusters of EPEC001 and EPEC080.

Orf no. Gene name Position of the gene G+C content (%) Similar protein(s), strain(s) (GenBank accession no.) %Identical/%Similar (total no. of aa) Putative function of protein
EPEC001
1 rmlB 1..1086 42.44 dTDP-glucose 4,6-dehydratase [Escherichia coli] (WP_052925278.1) 100/100 (361) dTDP-glucose 4,6-dehydratase
2 rmlD 1086..1985 47.55 dTDP-4-dehydrorhamnose reductase [Escherichia coli] (WP_046201417.1) 99/100 (299) dTDP-4-dehydrorhamnose reductase
3 rmlA 2043..2918 43.26 glucose-1-phosphate thymidylyltransferase RfbA [Escherichia coli] (WP_046201417.1) 99/100 (291) Glucose-1-phosphate thymidylyltransferase
4 rmlC 2927..3481 32.43 dTDP-4-dehydrorhamnose 3,5-epimerase [Escherichia coli] (WP_057080958.1) 99/100 (184) dTDP-4-dehydrorhamnose 3,5-epimerase
5 wzx 3490..4728 34.22 O34 family O-antigen flippase [Escherichia coli] (WP_097479960.1) 51/71 (412) flippase
6 glf 4731..5822 32.6 UDP-galactopyranose mutase [Escherichia coli] (WP_033560995.1) 75/85 (363) UDP-galactopyranose mutase
7 5825..6814 32.12 hypothetical protein [Escherichia coli] (WP_053273170.1) 99/99 (329) hypothetical protein
8 7459..8013 40.24 ISAs1 family transposase [Escherichia coli] (MBJ0238419.1) 96/97 (184) H repeat-associated protein
9 8068..8961 32.66 glycosyltransferase [Escherichia coli] (WP_085446706.1) 38/59(297) glycosyltransferase family 2 protein
10 wzy 9274..10335 29.75 EpsG family protein [Cronobacter muytjensii] (WP_075192411.1) 47/69 (353) polymerase
11 10345..11439 29.22 glycosyltransferase family 4 protein [Cronobacter muytjensii] (WP_083605367.1) 46/64 (364) glycosyltransferase
12 11436..12344 31.35 glycosyltransferase family 2 protein [Enterobacter asburiae] (WP_150182824.1) 62/77 (303) Galactofuranosyltransferase GlfT1
EPEC080
1 rmlB 1..1086 43.18 dTDP-glucose 4,6-dehydratase [Escherichia coli] (WP_029399178.1) 100/100 (361) dTDP-glucose 4,6-dehydratase
2 rmlD 1086..1985 46.11 dTDP-4-dehydrorhamnose reductase [Escherichia coli] (WP_029399176.1) 100/100(299) dTDP-4-dehydrorhamnose reductase
3 rmlA 2043..2921 43.34 glucose-1-phosphate thymidylyltransferase RfbA [Escherichia coli] (WP_029399175.1) 100/100 (292) Glucose-1-phosphate thymidylyltransferase 1
4 fdtA 2935..3354 32.85 FdtA/QdtA family cupin domain-containing protein [Cedecea lapagei] (WP_126355658.1) 66/83(139) TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase
5 fdtC 3332..3796 36.77 N-acetyltransferase [Escherichia coli] (EFN7827253.1) 100/100(154) dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase
6 fdtB 3801..4922 33.77 DegT/DnrJ/EryC1/StrS family aminotransferase [Escherichia coli] (HAO2821289.1) 99/99(373) dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose transaminase
7 wzx 4906..6177 30.47 O50 family O-antigen flippase [Escherichia coli] (EFN5080582.1) 54/74(423) flippase
8 6190..7221 31.2 glycosyltransferase family 4 protein [Enterobacter cloacae complex sp.] (WP_133294767.1) 53/71(343) glycosyltransferase family 4 protein
9 rmlC 7234..7767 34.08 dTDP-4-dehydrorhamnose 3,5-epimerase [Escherichia coli] (EEW2230532.1) 99/100(177) dTDP-4-dehydrorhamnose 3,5-epimerase
10 7793..8722 30.96 glycosyltransferase family 2 protein [Escherichia coli] (WP_063610376.1) 48/69(309) rhamnosyltransferase
11 wzy 8762..9805 27.2 EpsG family protein [Escherichia coli] (WP_089723541.1) 45/66(347 polymerase
12 9844..10599 28.04 glycosyl transferase group 2 family protein [Escherichia coli] (OAC41241.1) 58/76(251) UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-glucosyltransferase WfgD
13 10613..11722 30.21 glycosyltransferase [Croceivirga radicis] (WP_080317782.1) 52/71(369) Phosphatidyl-myo-inositol mannosyltransferase
14 manC 11738..13159 36.42 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase [Escherichia coli](WP_029399160.1) 100/100(473) Mannose-1-phosphate guanylyltransferase 1
15 manB 13180..14550 54.48 phosphomannomutase/phosphoglucomutase [Escherichia coli] (EFA9345916.1) 99/99(456) Phosphomannomutase/phosphoglucomutase
J. Microbiol. Biotechnol. 2021;31:1191~1199 https://doi.org/10.4014/jmb.2105.05016
© J. Microbiol. Biotechnol.