Fig. 2.

Fig. 2. CRISPR-mediated bacterial single base genome editing by target-mismatched and 5’-truncated sgRNAs. (A) Unedited target DNAs are cleaved by the gRNA/Cas9 complex. (B) Single base edited cells are also cleaved by the gRNA/Cas9 complex due to mismatch tolerance. Unedited target DNAs are still cleaved by the (C) target-mismatched or (E) 5’-truncated sgRNA/Cas9 complex. Mismatch intolerance of (D) target-mismatched or (F) 5’-truncated sgRNA/Cas9 complex enables bacterial single base genome editing (blue-shaded panels).

© J. Microbiol. Biotechnol.