Journal of Microbiology and Biotechnology

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1. Table 1

CRISPR/Cas-mediated pinpoint microbial base editing.

Edited length	Efficiency (%)	Cas system	Donor DNA	Organism	Target gene	Description	Reference
Single base	99.7	FnCpf1	Oligo	Corynebacterium glutamicum	crtEb	Target-mismatched crRNA	[98]
	95	SpCas9	Oligo	Escherichia coli	galK	Target-mismatched sgRNAs	[97]
	83	SpCas9	Oligo	E. coli	galK, xylB	5’-truncated sgRNAs	[99]
	68	SpdCas9-CBE	-	Agrobacterium tumefaciens	atu1060	Curing of the plasmid using sacB counter-selection	[101]
	65	SpCas9	Oligo	E. coli	rpsL	Single base substitution for streptomycin resistance acquisition	[42]
	30	SpCas9	Plasmid	Clostridium beijerinckii	pta	Two-step single nucleotide modification using artificial PAM or two-step indel	[60]
Double bases	100	SpCas9	dsOligo	Schizosaccharomyces pombe	swi6	Short-homology-mediated genome editing	[102]
	100	SpCas9	Oligo	Lactobacillus plantarum	rpoB	RecT-aided ssDNA recombineering	[58]
	100	AsCpf1	Oligo	C. glutamicum	crtYf	Comparison of 7 different Cas system	[103]
	100	FnCpf1	Oligo, PCR products	C. glutamicum	argR	RecT-aided ssDNA recombineering	[63]
	100	FnCpf1	Oligo	Zymomonas mobilis	ldh	Plasmid curing system using its native plasmid	[104]
	90	BbCpf1	Oligo	C. glutamicum	crtYf	Comparison of 7 different Cas system	[103]
	87.5	FnCpf1	Plasmid	E. coli	prpC	λ-red aided two-plasmid system	[56]
	76	FnCpf1	Oligo	E. coli	lacZ	Using X-Gal for blue-white colony screening	[55]
	60	FnCpf1	Oligo	Mycobacterium smegmatis	ms1521	λ-red aided ssDNA recombineering	[55]
	60	TsCpf1	Oligo	C. glutamicum	crtYf	Comparison of 7 different Cas system	[103]
Triple bases	100	SpCas9	Plasmid	Bacillus subtilis	trpC	Substitution of PAM sequence	[53]
	100	SpCas9	Oligo	Streptococcus pneumoniae	bgaA	Amino acid change and creating BtgZⅠ site	[42]
	94 ~ 99	SpCas9	Oligo, PCR products	E. coli	rpoB, ack	λ-red aided ssDNA recombineering	[57]
	70 ~ 86.7	SpCas9	Oligo	C. glutamicum	rpsL, rfp	Editing of streptomycin resistance phenotype or integrated fluorescence gene	[51]
	71	SpCas9	Oligo	L. plantarum	glmS1	Overexpression of Dam to interfere MMR	[105]
Quadruple bases	47	FnCpf1	Oligo	C. glutamicum	mscCG	Site-directed mutagenesis using a random mutagenic oligo	[106]
	17	Synthetic Cas9	PCR products	Ustilago maydis	cdk5	Introduction of point mutations using two-step PCR with three DNA fragments	[107]

J. Microbiol. Biotechnol. 2021;31:903~911 https://doi.org/10.4014/jmb.2106.06056