Fig. 2. Target DNA detection with engineered ZFPs on a magnetic bead-based platform.
(A, C) The bar graph representing the fluorescence intensity of individual particles obtained from (B, D) images taken by fluorescence microscopy (i: 0 nM, ii: 4 nM, iii: 20 nM, iv: 100 nM, v: 500 nM, and vi: 2500 nM DNA). (A, B) Alexa 488-labeled target DNA was detected by ZFP Stx2-268. (C, D) Texas red-labeled target DNA was detected by ZFP SEB-435.