Fig. 7.

Fig. 7. E2F7 was a target gene of miR-3127-5p. (A) The miRWalk (http://mirwalk.umm.uni-heidelberg.de/), miRDB (http://mirdb.org/), starBase, Targetscan (http://www.targetscan.org/vert_72/docs/help.html) were used to predict miR-3127- 5p target genes. The Cancer Genome Atlas (TCGA) database (http://cancergenome.nih.gov/) analyzed mRNAs that are abnormal expressed in rectal adenocarcinoma. Venn diagram was used to analyze the intersection of the data generated from the databases. (B-C) SHBG and E2F7 expressions were detected by RT-qPCR. (D-F) E2F7 bound to miR-3127-5p as predicted and verified by Targetscan and dual luciferase assay. ⁺⁺p < 0.01, ⁺⁺⁺p < 0.001 vs MC.

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