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Fig. 5.

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Fig. 5. LINC00174 affected the proliferation of colorectal cancer (CRC) cells through miR-3127-5p. (A) The binding site of LINC00174 and miR-3127-5p was predicted using the starBase website. (B-E) Dual-luciferase report analysis and RNA immunoprecipitation verified the targeting relationship between LINC00174 and miR-3127-5p. (F-G) The transfection rate of miR-3127-5p mimic transfected SW480 or LOVO cells was detected by RT-qPCR. U6 was internal reference. (H-I) The expression of miR-3127-5p in Control, LINC00174, LINC00174+miR-3127-5p mimic control, LINC00174+miR-3127-5p mimic, miR-3127-5p mimic group was detected by RT-qPCR. U6 was internal reference. (J-M) Clone formation experiments were used to detect the proliferation of SW480 or LOVO cells in each group. ++p < 0.01, +++p < 0.001 vs miR-3127-5p mimic control (MC); &&p < 0.01, &&&p < 0.001 vs Anti-lgG; **p < 0.01, ***p < 0.001 vs Control; #p < 0.05, ##p < 0.01, ###p < 0.001 vs LINC00174; ^^p < 0.01, ^^^p < 0.001 vs M.
J. Microbiol. Biotechnol. 2021;31:1098~1108
© J. Microbiol. Biotechnol.