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Fig. 3.

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Fig. 3. SiHSP70 reversed the effect of SPostC on HSP70 expression, LDH level, apoptosis, and cell viability in HR-induced H9c2 cells. (A) The level of HSP70 in H9c2 cells, which were transfected with siHSP70 and then treated with HR or SPostC, was assessed by RT-qPCR. Mean band density was normalized relative to GAPDH. (B) The level of HSP70 in H9c2 cells, which were transfected with siHSP70 and then treated with HR or SPostC, was assessed by Western blotting. Mean band density was normalized relative to GAPDH. (C) The level of LDH in H9c2 cells, which were transfected with siHSP70 and then treated with HR or SPostC, was assessed by colorimetry. (D) Cell viability of H9c2 cells, which were transfected with siHSP70 and then treated with HR or SPostC, was assessed by MTT assay. (E) Apoptosis of H9c2 cells subjected to HR injury or SPostC with low expression of HSP70 was assessed by flow cytometry. ***p < 0.001, vs. Control; ##p < 0.01, ###p < 0.001, vs. HR; ^^^p < 0.001, vs. siNC; ▲▲▲p < 0.001, vs. siHSP70; △△△p < 0.001, vs. SPostC. (HR: hypoxic/reoxygenation; SPostC: sevoflurane postconditioning; HSP70: heat shock protein 70; LDH: lactate dehydrogenase; MTT: methyl thiazolyl tetrazolium; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; siNC: small interfering RNA targeted negative control; siHSP70: small interfering RNA targeted HSP70).
J. Microbiol. Biotechnol. 2021;31:1069~1078 https://doi.org/10.4014/jmb.2103.03040
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