Fig. 4.

Fig. 4. Rg4 upregulates hair-inducing activity via WNT/β-catenin signaling pathway in DP spheres. (A) β- catenin translocation was analyzed by immunoblotting assay in Rg4 treatment DP spheres. Lamin A/C and β-tubulin were used as loading control for total protein. (B) The luciferase activity of TCF/LEF reporter plasmid was confirmed by luciferase assay. DP cells were treated with Rg4 (20 and 50 μg/ml) with or without LY294002 (20 μM) for 48 hours. The luciferase assay was determined by normalizing the β-galactosidase activity. (C) The mRNA expression level of target genes including WNT5A, β-catenin, and LEF1 were confirmed by RT-qPCR analysis. DP cells were treated with the indicated doses of Rg4 (20 and 50 μg/ml) for 48 h. The results are presented as the mean ± SD of three independent experiments. Values of *p < 0.05 were considered to be statistically significant.

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