List of sequences used for RT-qPCR.
Targets of RT-qPCR | Sequence of primer |
---|---|
Bone morphogenetic protein 2 ( |
F : 5’- GGAACGGACATTCGGTCCTT -3’ |
R : 5’- CACCATGGTCGACCTTTAGGA -3’ | |
Versican ( |
F : 5’- TGTCCGATTCATAGTCCTGTC -3’ |
R : 5’- CTCACAGCGATAAGTGCCCTC -3’ | |
Alkaline phosphatase ( |
F : 5’- CAAACCGAGATACAAGCACTC -3’ |
R : 5’- CGAAGAGACCCAATAGGTAGTCCAC -3’ | |
WNT family member 5A ( |
F : 5’- TTGAAGCCAATTCTTGGTGGTCGC -3’ |
R : 5’- TGGTCCTGATACAAGTGGCACAGT -3’ | |
Lymphoid enhancer binding factor 1 ( |
F : 5’- GCTGTCTTTCTTTCCGTGCTA -3’ |
R: 5’- GCTGTCTTTCTTTCCGTGCTA -3’ | |
β-catenin | F : 5’- CCCACTAATGTCCAGCGTTT -3’ |
R : 5’- AACCAAGCATTTTCACCAGG -3’ | |
Glyceraldehyde-3-phosphate dehydrogenase ( |
F : 5’- CGGAGTCAACGGATTTGGTCGTAT -3’ |
R : 5’- AGCCTTCTCCATGGTGGTGAAGAC -3’ |