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Fig. 4.

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Fig. 4. CRLS1 functioned as a target of miR-7-5p. (A) The number of potential target genes for miR-7-5p from StarBase, miRDB, TargetScan, miRWalk and miRTarbase and TCGA-LUAD database. (B) The Venn diagram of potential target genes for miR-7-5p. (C) The binding sites between miR-7-5p and CRLS1. (D and E) Relative luciferase activity of A549 (D) and PC-9 (E) cells was assessed through dual-luciferase reporter analysis after transfection of CRLS1-WT and miR-7-5p mimic. (F) Relative miR-7-5p expression between LC and adjacent normal tissues was evaluated by qRT-PCR. (G) Relative CRLS1 expression between LC and adjacent normal tissues was measured through qRT-PCR. ***p < 0.001 vs. MC group. All experiments were repeated independently at least three times. Data were performed as the means ± SD. Abbreviations: CRLS1, cardiolipin synthase 1; TCGA-LUAD, The Cancer Genome Atlas Lung Adenocarcinoma; CRLS1-WT, wild-type CRLS1; LC, lung cancer; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; MC, miR-7-5p mimic control.
J. Microbiol. Biotechnol. 2021;31:921~932 https://doi.org/10.4014/jmb.2102.02010
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