Primer sequences used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
Target gene | Primers, 5’-3’ |
---|---|
IL-6 | |
(Forward) | ACTCACCTCTTCAGAACGAATTG |
(Reverse) | CCATCTTTGGAAGGTTCAGGTTG |
IL-8 | |
(Forward) | GTGCATAAAGACATACTCCA |
(Reverse) | CTCTTCAAAAACTTCTCCAC |
TNF-α | |
(Forward) | CCTCTCTCTAATCAGCCCTCTG |
(Reverse) | GAGGACCTGGGAGTAGATGAG |
Nrf2 | |
(Forward) | TCAGCGACGGAAAGAGTATGA |
(Reverse) | CCACTGGTTTCTGACTGGATGT |
β-actin | |
(Forward) | CTCCATCCTGGCCTCGCTGT |
(Reverse) | GCTGTCACCTTCACCGTTCC |