Fig. 2.

Fig. 2. Cytotoxic mitochondrial targeting domain (MTD) homologs induce intracellular calcium influx. HeLa cells were incubated with calcium indicator Fluo-4-AM (5 μM) for 10 min before treatment with cytotoxic mitochondrial targeting domain homologs fused with octa-arginine (R8) to visualize intracellular calcium concentration. After treatment with R8:M-SDAD1 (5 μM, A), R8:M-TRRAD (20 μM, B), and R8:M-ST3GAL3 (40 μM, C), the intensity of Fluo-4 was measured using confocal microscopy (one picture every 5 s) with 488 nm argon laser. Cell morphology was examined using differential interference contrast microscopy.

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