JMB

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Table. 2.

Table. 2.

Primers used for reverse transcription PCR.

PCR reaction Genes name Primer pairs Sequences Expected size
RT-PCR aprE3-5 aprE-RT-F
aprE-RT-R
5'-TGGATCAGCTTGTTGTTTGCG-3'
5'-GGGTGCTTAGAAAGGATTAGC-3'
1 kb
16S rRNA 27F
1492R
5'-AGAGTTTGATCCTGGCTCAG-3'
5'-GGTTACCTTGTTACGACTT-3'
1.5 kb
qRT-PCR aprE3-5 aprE-qRT-F
aprE-qRT-R
5'-AACAGCAGCAACCAAAGAGC-3'
5'-TCGGGTGCTTAGAAAGGAT-3'
178 bp
16S rRNA 16S-qRT-F
16S-qRT-R
5'-GAGTGACAGCTGGTGCATGGT-3'
5'-TTGTCACCGGCAGTCACCTTA-3'
160 bp
J. Microbiol. Biotechnol. 2021;31:833~839 https://doi.org/10.4014/jmb.2103.03027
© J. Microbiol. Biotechnol.