Fig. 3.

Fig. 3. Circ_0000144 directly sponged miR-217. (A) The complementary binding site of miR-217 and circ_0000144. (B) Luciferase activity of circ_0000144-WT and circ_0000144-MUT after co-transfection with miR-217 inhibitor or NC in AGS and NCI-N87 cells. (C) The potential targets of miR-217 were predicted by TargetScan, miRDB, miRwalk, starBase and DEGS from TCGA-STAD database. (D and E) Luciferase report assay was performed to verify the interaction between ANLN and miR-217. (F) RNA pulldown confirmed the interaction between ANLN and miR-217. The experiment was independently conducted three times. **p < 0.01, ***p < 0.001 vs. Blank; ^###p < 0.001 vs. mimic control; ^^^p < 0.001 vs. Bio-NC. WT: wild type; MUT: mutant type; GC: gastric cancer.

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