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Fig. 5.

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Fig. 5. DPT reduces a mitochondrial membrane potential and induces caspase activity in HCC827GR cells. HCC827GR cells were treated with different concentrations of DPT and incubated for 48 h. (A-B) The movements of fluorescence from right to left indicate depolarization of MMP. The mitochondrial membrane potential was evaluated by Muse MMP kit. Results are expressed as mean ± SD of three independent experiments. (C) CHOP, DRs and CHOP proteins were probed in western blotting. (D) After treatment of DPT, the proteins were detected by specific antibodies. The expression levels of Bid, Bcl-xl, Mcl-1, Bad, cyto C (cytosol), α-tubulin (cytosol), cyto C (mitochondria), COX 4 (mitochondria), Apaf-1, PARP and cleaved-PARP were normalized to actin. (E-F) The plots depict the efficacy of DPT and GEF treatments in the lung cancer cell indicated. The cells were treated with DPT at various concentrations or GEF for 24 h. Caspase activity was measured by Muse Cell Analyzer. The data are representative of three experiments independently. (G) HCC827GR cells were pretreated with 8 μM of pan-caspase inhibitor that named Z-VAD-FMK for 3h and treated DPT (8 nM) indicated. Statistically significant results are represented as *p < 0.05, remarkably different from DPT-untreated control cells, #p < 0.05, remarkably different from DPT-treated cells.
J. Microbiol. Biotechnol. 2021;31:559~569 https://doi.org/10.4014/jmb.2101.01029
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