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Fig. 3.

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Fig. 3. DPT inhibits cell viability and induces apoptosis and cell cycle arrest in HCC827GR cells. (A) MTT assay shows the effect of DPT on HCC827GR cell viability. Cells were treated with varying concentration of chemicals for 24 h or 48 h (DPT concentrations: 4, 6, 8 nM, GEF concentration: 1 μM). The cell viability in lung cancer cells decreased in dosedependent chemical that was measured by MTT assay. Data was representative of three independent experiments and expressed as mean ± SD. *p < 0.05 represent statistical difference between control and DPT or GEF test groups. (B) HCC827GR cells were seeded in soft agar and treated with DPT or GEF. The colonies were pictured after 2 weeks by light microscope. (C) The colonies were counted as the number of rates of apoptotic cells from triplicate independent experiments and the result was mean ± SD of three experiments (*p < 0.05). (D-E) HCC827GR cells were treated with increasing concentrations of DPT or GEF for 48 h. Annexin V and PI-stained HCC827GR cells were accessed by Muse cell analyzer. The result represented by 4 different populations of cells: live cells (lower left quadrant), early apoptotic cells (lower right quadrant), late apoptotic/dead cells (upper right quadrant) and necrotic/dead cells (upper left quadrant). (F) Whole cell lysate was analyzed by Westernblotting with antibodies against cyclin B1, cdc2, p21 and actin. (G-I) HCC827GR cells were exposed to DPT or GEF for 48 h. Cell cycle progression and sub G1 population of HCC827GR cells following chemical treatment were examined by Muse cell analyzer. Three experiments were conducted independently, and values represent means ± SD (*p < 0.05, compared with control).
J. Microbiol. Biotechnol. 2021;31:559~569 https://doi.org/10.4014/jmb.2101.01029
© J. Microbiol. Biotechnol.