JMB

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Table. 3.

Table. 3.

Optimization of MCLSA assay for detection of HPV-16.

Conditions The test interval Difference Good yield Final selecteda
Temperature 60 - 70 ℃ 2 ℃ 62 - 68 ℃ 62 ℃
MgCl2 1.0 - 3.0 mM 1.0 mM 2.0 - 3.0 mM 2.0 mM
dNTPs 1.0 - 4.0 mM 1.0 mM 2.0 mM 2.0 mM
Betaine 0.6 - 1.2 M 0.2 M 1.0 M 1.0 M
Bst polymerase 6 - 12 U 2 U 8 U 8 U
Incubation time 30 - 120 min 15 min 45 - 120 min 45 min

aThe final concentrations of reagents or temperature and times used in MCLSA assay.

J. Microbiol. Biotechnol. 2021;31:610~620 https://doi.org/10.4014/jmb.2012.12047
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