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Fig. 5.

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Fig. 5. Hyperbolic fit for progesterone. The reaction was catalyzed by CYP154C3-1 (A) and CYP154C3-2 (B) using Pdx-Pdr-NADH. The reaction mixture contained CYP: Pdx: Pdr at a ratio of 1: 8: 2 with varied substrate concentration and the reaction was started by the addition of 250 μM NADH. The graph of rate of reaction vs substrate concentration was plotted. Data were analyzed by non-linear regression analysis based on Michaelis-Menten kinetics. The values are the mean of three independent experiments with standard deviation.
J. Microbiol. Biotechnol. 2021;31:464~474
© J. Microbiol. Biotechnol.