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Fig. 4.

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Fig. 4. Optimum pH and temperature and the effect of ionic strength on CYP154C3-1 (■) and CYP154C3-2 (●). (A) Optimum pH. The CYP154C3-1 and CYP154C3-2 activities were measured over a pH range of 6.0–8.5 at 30°C for 1 h. Both the CYPs favored alkaline pH (7.0–7.8), retaining more than 92% of the maximal activity. (B) Optimum temperature. The activity of reactions at 15–50°C in pH 7.4 was measured for 1 h. At a 30–37°C temperature, the enzyme retained more than 98% of maximal activity. (C) The effect of ionic strength on the catalytic activity of the enzymes in various ionic strength solutions maintained by NaCl (10–200 mM). A bell-shaped curve revealed improvement in activity in lower ionic strength, while higher ionic strength (>70 mM) decrease the enzyme activity. The hydroxylation of progesterone was measured. The values are the mean of three independent experiments with standard deviation.
J. Microbiol. Biotechnol. 2021;31:464~474
© J. Microbiol. Biotechnol.