JMB

eISSN 1738-8872
pISSN 1017-7825

Table. 3.

Table. 3.

Comparison of the N-gene targeting SARS-CoV-2 qPCR and ddPCR assays.

Dilution factor 2019-nCoV_N1 2019-nCoV_N2 NIID_2019-nCoV_N WH-NIC N HKU-N

qPCR ddPCR qPCR ddPCR qPCR ddPCR qPCR ddPCR qPCR ddPCR
10-2 18.89 S* 18.87 S 18.14 S 21.40 S 20.79 S
10-3 22.16 21173 22.19 20340 21.63 15173 24.65 19993 24.31 18473
10-4 25.99 1907 26.06 1659 25.40 1177 28.52 1599 28.09 1553
10-5 30.66 135 30.60 132 29.93 60 33.11 105 32.68 105
10-6 34.12 11 34.57 8 34.91 7 36.71 5 36.71 12
R2 0.9968 0.9973 0.9943 0.9971 0.9978

*Saturated

**All template were performed in triplicate and data in table indicates average value.

J. Microbiol. Biotechnol. 2021;31:358~367 https://doi.org/10.4014/jmb.2009.09006
© J. Microbiol. Biotechnol.