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Table. 2.

Table. 2.

Validation of the SARS-CoV-2 specific N gene targeting primer-probe sets used in the study.

Template SARS-CoV-2 (15 pg/μl) SARS-CoV (8.7 pg/μl) MERS-CoV (72 pg/μl) HCoV-OC43 (60 pg/μl)

Primer-probes qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl)
2019-nCoV_N1 26.30 6123 Undetermined 0 Undetermined 0 Undetermined 0
2019-nCoV_N2 26.57 5340 Undetermined 2.2 Undetermined 4.3 39.09 4.8
NIID_2019-nCoV_N 26.89 3993 Undetermined 0 Undetermined 0 Undetermined 0
HKU-N 27.57 4863 27.24 6030 Undetermined 0 Undetermined 0.8
WH-NIC N 28.91 6153 Undetermined 0 Undetermined 0 Undetermined 0.5

*All template were performed in triplicate and data in table indicates average value.

J. Microbiol. Biotechnol. 2021;31:358~367 https://doi.org/10.4014/jmb.2009.09006
© J. Microbiol. Biotechnol.