JMB

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Table. 5.

Table. 5.

Results of the Ct values by qPCR and copy number by ddPCR on patient sample.

Primer-probe 2019-nCoV_N2 primer-probe set

Template P1 P2 P3 P4 P5

Dilution factor qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl)

10-1 26.87 1051 35.03 7.2 35.15 2.4 Undetermined 0 35.05 7.53
10-2 30.52 82 37.03 1.33 Undetermined 0.73 Undetermined 0.53 Undetermined 1.2
10-3 33.81 5.93 Undetermined 0.67 Undetermined 0.6 Undetermined 0 Undetermined 1.07

Primer-probe WH-NIC N primer-probe set

Template P1 P2 P3 P4 P5

Dilution factor qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl) qPCR (Ct) ddPCR (copy/μl)

10-1 28.77 836.00 36.11 5 35.88 6.80 Undetermined 1.40 35.86 7.67
10-2 32.25 107.33 38.54 3 38.52 1.93 Undetermined 0.00 37.02 1.87
10-3 35.95 10.40 Undetermined 2.40 38.27 2.07 38.44 1.27 Undetermined 0.47

*The initial concentration of RNA templates were 46.9, 21.1, 27.5, 17.2, and 15.9 ng/μl, respectively.

**All template were performed in triplicate and data in table indicates average value.

+Only one of the triplicate was positive (single droplet)

J. Microbiol. Biotechnol. 2021;31:358~367 https://doi.org/10.4014/jmb.2009.09006
© J. Microbiol. Biotechnol.