Fig. 3.

Fig. 3. TRAF6 was a target gene of miR-361-3p and the effects of TRAF6 on cell viability. (A) TRAF6 was the possible target genes of miR-361-3p, predicted by TargetScan. (B-C) Dual-luciferase reporter assay results revealed that the miR-361-3p mimic reduced luciferase activity of TRAF6 -WT. **p < 0.001 vs. Blank. (D-F) The WB and qRT-PCR analysis result showed that TRAF6 was more up regulated in plasma of MM patients than that of normal controls. **p < 0.001 vs. Normal. ##p < 0.001 vs. normal 2. (G-H) The qRT-PCR analysis was applied to show the increased TRAF6 expression levels after TRAF6 overexpression transfection. (I-J) The qRT-PCR analysis result showed that overexpression of miR-361-3p down regulated the level of TRAF6. (K-L) The CCK-8 assay results revealed that TRAF6 overexpression increased cell viability, which partly reversed the inhibitory effect of miR-361-3p overexpression on MM cells. &&p < 0.001 vs. NC; **p < 0.001 vs. MC+NC; ##p < 0.001 vs. MC+TRAF6; ^^p < 0.001 vs. mimic+NC. MC was mimic control, NC was the empty control for TRAF6.

© J. Microbiol. Biotechnol.