JMB

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1.

Plasmids used in this study.

Plasmid name Description References
pX330 Streptococcus pyogenes Cas9, Amp [23-24]
pYPZ37 URA3, AfRibo, wA flanking, Amp, Pfma cluster (PfmaA-E, PfmaG, gpdA(p)::PfmaH) [25]
pET-28a Protein expression vector Invitrogen
pCC1BAC BAC vector [21]
pYJF22 S. pyogenes Cas9, Kan This study
pYJF24 URA3, PyrG, Cm, wA flanking in pCC1BAC- This study
pYJF26 URA3, AfRibo, Cm, wA flanking in pCC1BAC This study
pYJF27 Nrc-5F and 3F in pYJF24 This study
pYJF28 7101-5F and 3F in pYJF26 This study
pYJF31 Nrc-Cluster in pYJF27 This study
J. Microbiol. Biotechnol. 2021;31:8~15 https://doi.org/10.4014/jmb.2008.08040
© J. Microbiol. Biotechnol.