Fig. 5.

Fig. 5. TMEE suppresses the nucleus translocation of NF-κB in LPS-stimulated RAW264.7 cells. (A) The protein level of NF-κB p65 in the nucleus was determined using western blot analysis. (B) The nuclear translocation of NF-κB was measured using immunocytochemistry. Cells were observed under a confocal microscope and images were captured using LSM 510 Meta software. Scale bar = 30 μm. The effects of a combination of TMEE and NF-κB inhibitor on (C) NF-κB nuclear translocation and (D) NO production in LPS-stimulated RAW264.7 cells. #p < 0.05 vs. negative control group; *p < 0.05 and **p < 0.01 vs. LPS only group; †p < 0.05 vs. TMEE only or BAY11-7085 group. PCNA, proliferating cell nuclear antigen. LPS + T40, LPS + TMEE 40 μg/ml; TMEE, Trichilia martiana C. DC ethanol extract; LPS, lipopolysaccharide; NO, nitrous oxide.

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