Fig. 5. Intestinal microbiota richness and diversity was increased with prebiotic treatment. (A) Richness, (B) Diversity, and (C) Principal coordinate analysis (PcoA) of community structures using a Fast UniFrac distance matrix. C: Untreated control, N: AD control (DNCB-induced), P: Zyrtec-positive control (DNCB-induced + Zyrtec), T1: scGOS/lcFOS (DNCB-induced + scGOS/lcFOS), T2: Inulin (DNCB-induced + Inulin), T3: β-glucan (DNCB-induced + β-glucan). Percentage of variation explained by principal component 1 (PC1): 46.726%; percentage of variation explained by principal component 2 (PC2): 14.962%; percentage of variation explained by principal component 3 (PC3): 6.865%. *p < 0.05 versus control group, **p < 0.01 versus control group, #p < 0.05 versus negative control group, ##p < 0.01 versus negative control group. Data represent means ± SD of 4 replicates.