Fig. 4. Prebiotics increase TLR-9 and Gal-9 protein expression in HT29 cell lines (A, B, C). Western blot analysis showed a dose-dependent increase in TLR-9 and Gal-9 protein levels in HT29 cells following treatment with scGOS/lcFOS (9:1 w/v mixture), inulin, and β-glucan for 12 h, respectively.