JMB

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Table. 2.

Table. 2.

qPCR primer sequences for the analysis of beneficial bacteria.

Target microorganism Primer Reference
Universal Forward 5’ GTGSTGCAYGGYYGTCGTCA 3’ 20
Reverse 5’ ACGTCRTCCMCNCCTTCCTC 3’
Ruminococcus spp. Forward 5’ GGCGGCYTRCTGGGCTTT 3’ 21
Reverse 5’ CCAGGTGGATWACTTATTGTGTTAA 3’
Roseburia spp. Forward 5’ GCGGTRCGGCAAGTCTGA 3’ 21
Reverse 5’ CCTCCGACACTCTAGTMCGAC 3’
Faecalibacterium prausnitzii Forward 5’ GGAGGAAGAAGGTCTTCGG 3’ 21
Reverse 5’ AATTCCGCCTACCTCTGCACT 3’
Bifidobacterium spp. Forward 5’ TCGCGTCYGGTGTGAAAG 3’ 22
Reverse 5’ GGTGTTCTTCCCGATATCTACA 3’
Lactobacillus sakei Forward 5’ CCATGTGTAGCGGTGAAATG 3’ 23
Reverse 5’ ATCCTGTTTGCTACCCATGC 3’
Leuconostoc mesenteroides Forward 5’ TGATGCATAGCCGAGTTGAG 3’ 23
Reverse 5’ GAAAGCCTTCATCACACACG 3’
Leuconostoc citreum Forward 5’ GGAAACAGATGCTAATACCGAATA 3’ 23
Reverse 5’ TTTACCCCACCAACTAACTAATG 3’
Weissella cibaria Forward 5’ GGGAAACCTACCTCTTAGCA 3’ 23
Reverse 5’ GGACCATCTCTTAGTGATAGCA 3’
Weissella koreensis Forward 5’ GGGCTACACACGTGCTACAA 3’ 23
Reverse 5’ GATTCCGACTTCGTGTAGGC 3’
J. Microbiol. Biotechnol. 2020;30:1343~1354 https://doi.org/10.4014/jmb.2005.05017
© J. Microbiol. Biotechnol.