Fig. 2. Effect of prebiotics on the expression of transcription factors and cytokines. T-cell polarization in mesenteric lymph nodes (MLN) was evaluated by analyzing the expression of T-bet, IFN-γ (Th1, A), GATA-3, IL-4 (Th2, B), RORγT, IL-17 (Th17, C) and Foxp3, TGF-β (Treg, D). C: untreated control, N: AD control (DNCB-induced), P: Zyrtec-positive control (DNCB-induced + Zyrtec), T1: scGOS/lcFOS (DNCB-induced + scGOS/lcFOS), T2: Inulin (DNCB-induced + Inulin), T3: β-glucan (DNCB-induced + β-glucan); *p < 0.05 versus control group, **p < 0.01 versus control group, #p < 0.05 versus negative control group, ##p < 0.01 versus negative control group. Data represent means ± SD of 4 replicates.