eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 1.
Table. 1.
RT-PCR primer sequences for the analysis of immunomodulatory genes.
| Target gene | Primer | Reference | |
|---|---|---|---|
| GAPDH | Forward | 5’ CCACCCAGAAGACTGTGGAT 3’ | 15 |
| Reverse | 5’ CACATTGGGGGTAGGAACAC 3’ | ||
| T-bet | Forward | 5’ TCAACCAGCACCAGACAGAG 3’ | 16 |
| Reverse | 5’ AAACATCCTGTAATGGCTTGTG 3’ | ||
| GATA-3 | Forward | 5’ CATTACCACCTATCCGCCCTATG 3’ | 16 |
| Reverse | 5’ CACACACTCCCTGCCTTCTGT 3’ | ||
| RORγT | Forward | 5’ TTCACCCCACCTCCACTG 3’ | 16 |
| Reverse | 5’ TGCAAGGGATCACTTCAATTT 3’ | ||
| Foxp3 | Forward | 5’ CCCATCCCCAGGAGTCTTG 3’ | 17 |
| Reverse | 5’ CCATGACTAGGGGCACTGTA 3’ | ||
| INF-γ | Forward | 5’ TCAAGTGGCATAGATGTGGAAGAA 3’ | 17 |
| Reverse | 5’ TGGCTCTGCAGGATTTTCATG 3’ | ||
| IL-4 | Forward | 5’ ACAGGAGAAGGGACGCCAT 3’ | 17 |
| Reverse | 5’ GAAGCCCTACAGACGAGCTCA 3’ | ||
| IL-17 | Forward | 5’ TTCATCTGTGTCTCTGATGCT 3’ | 17 |
| Reverse | 5’ TTGACCTTCACATTCTGGAG 3’ | ||
| TGF-β | Forward | 5’ GAAGGCAGAGTTCAGGGTCTT 3’ | 17 |
| Reverse | 5’ GGTTCCTGTCTTTGTGGTGAA 3’ | ||
| Galectin-9 | Forward | 5’ GAGAGGAAGACACACATGCCTTTC 3’ | 18 |
| Reverse | 5’ GACCACAGCATTCTCATCAAAACG 3’ | ||
| Filaggrin | Forward | 5’ CACTGAGCAAAGAAGAGCTGAA 3’ | 19 |
| Reverse | 5’ CGATGTCTTGGTCATCTGGA 3’ | ||
| TSLP | Forward | 5’ AGAGAAGCCCTCAATGACCAT 3’ | 19 |
| Reverse | 5’ GGACTTCTGTGCCATTTCC 3’ |
J. Microbiol. Biotechnol. 2020;30:1343~1354 https://doi.org/10.4014/jmb.2005.05017
© J. Microbiol. Biotechnol.