Fig. 4.

Fig. 4. Effect of acacetin on UVB-induced MMP-1 protein expression in HaCaT cells and human dermal fibroblasts. (A) HaCaT cells were pretreated with acacetin at indicated concentrations for 1 h and then further treated with 0.01 J/cm² UVB for 48 h at 37°C. (C) MMP-1 protein expression data of HaCaT cells were quantified using NIH ImageJ software. Data (n = 3) represent the mean ± SD. (B) HDF were pretreated with acacetin at indicated concentrations for 1 h and then further treated with 0.02 J/cm² UVB for 48 h at 37°C. (E) MMP-1 protein expression data of HDF were quantified using the NIH ImageJ software. Data (n = 3) represent the mean ± SD. (C) Cell viability of acacetin was measured using MTT assay. Cells were starved in serum-free DMEM for 24 h. Cells and acacetin were then incubated at 37°C for 22 h followed by treatment with MTT solution for 2 h. Data (n = 5) represent the mean ± SD. The experiment was performed in triplicate and bars marked with different letters (a–c) are significantly different (p < 0.05, ##p < 0.01), relative to the control cells.

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