Fig. 2.

Fig. 2. Effect of safflower seed oil (SSO) on UVB-induced MMP-1 protein expression in HaCaT cells and human dermal fibroblasts. (A) HaCaT cells were pretreated with SSO at indicated concentrations for 1 h and further treated with 0.01 J/cm² UVB for 48 h at 37°C. (B) Human skin fibroblasts (HDF) were pretreated with SSO at indicated concentrations for 1 h and further treated with 0.02 J/cm² UVB for 48 h at 37°C. MMP-1 protein expression data of HDF were quantified using NIH ImageJ software. Data (n = 3) are presented as mean ± SD. (C) Cell viability of SSO was determined using MTT assay as described in Materials and Methods. Data (n = 5) shown are presented as means ± SD. #p < 0.05 and ##p < 0.01 relative to control cells. *p < 0.05 and **p < 0.01 relative to UVB-induced cells. The experiment was performed in triplicate and bars marked with different letters (a–c) are significantly different (p < 0.05, ##p < 0.01), relative to the control cells.

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