Fig. 5. Inhibitory effect of periplanetasin-5 on MAPKs and NF-kB signaling pathways in Raw264.7 cells. (A) Raw264.7 cells were incubated with the indicated concentration of periplanetasin-5. Proteins were then isolated 30 min after LPS treatment, and phosphorylation of ERK, p38, and JNK was detected through western blot analysis. (B) Phosphorylation and degradation of IkB were detected through western blot analysis. CTR, control; LPS, lipopolysaccharide.