Primer sequences for real-time PCR.
cDNAs | Primer sequence | Accession No. |
---|---|---|
Cyclooxygenase-2 (COX-2) | Forward, 5'-CAGACAACATAAACTGCGCCTT-3' | NM_011198 |
Reverse, 5'-GATACACCTCTCCACCAATGACC-3' | ||
Inducible nitric oxide synthase (iNOS) | Forward, 5'-CAGCACAGGAAATGTTTCAGC-3' | NM_010927 |
Reverse, 5'-TAGCCAGCGTACCGGATGA-3' | ||
Iinterleukin-6(IL-6) | Forward, 5'-GAGGATACCACTCCCAACAGACC-3' | NM_031168 |
Reverse, 5'-AAGTGCATCATCGTTGTTCATACA-3' | ||
Tumor necrosis factor-α (TNF-α) | Forward, 5'-ATGAGAAGTTCCCAAATGGC-3' | NM_013693 |
Reverse, 5'-CTCCACTTGGTGGTTTGCTA-3' | ||
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) | Forward, 5'-AAGGTCATCCCAGAGCTGAA-3' | NM_008084 |
Reverse, 5'-CTGCTTCACCACCTTCTTGA-3' |