Fig. 4.

Fig. 4. Requirement of MRS3/4 for virulence in a mouse inhalation model. (A) Ten female BALB/c mice were intranasally infected with each of the strains indicated, and mouse survival was monitored twice per day. The results from the assays indicate that MRS3/4 is required for full virulence. (B) The distribution of fungal cells in the organs (blood, kidney, spleen, brain, lung, and liver) of infected mice. Organs from wild-type and mrs3/4 mutant infected mice were collected at the humane endpoint of the experiment, and fungal burdens were quantified by CFUs. In all organs, differences in fungal burden between the wild-type and the mrs3/4 mutant were statistically significant, as assessed by a nonparametric two-tailed Mann- Whitney U test (*p < 0.05; **p < 0.001).

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