Fig. 3.

Fig. 3. Mitochondrial localization of the Mrs3/4 protein and impaired mitochondrial iron metabolism in the mrs3/4 mutant. (A)) The strain expressing the Mrs3/4-GFP fusion protein was stained with 100 nM of Mitotracker to visualize mitochondria. The scale bar represents 5 μm. Iron contents of isolated mitochondria (B) and total intracellular heme contents (C) were determined by colorimetric assays. Values indicate iron or heme contents relative to those of the wild-type and represent the average from three independent experiments, with standard deviations. (D) The activity of aconitase was determined using in-gel assays, and the intensity of each band was quantified. CPTA (copper phthalocyanine-3, 4', 4", 4'"- tetrasulfonic acid tetrasodium) shows equal sample loading. All experiments were carried out in triplicate.

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