Fig. 2.

Fig. 2. Iron transport and metabolism deficiencies in the mrs3/4 mutant. (A) The growth of the mrs3/4 mutant in low-iron YNB media (LIM) containing various iron sources was monitored. Ten-fold serial dilutions of cells (starting at 104 cells) were spotted onto the plates and incubated at 30°C for 2 days. (B) The transcript levels of CFO1, FRE2, and SIT1 were determined using qRT-PCR. Data were normalized using TEF2 as an internal control and represent the average from three independent experiments (with standard deviations indicated). (C) Measurement of ferric reductase activity was carried out using a TCC overlay assay. TCC was poured on spotted cells and plates were photographed after 10 min.

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