Fig. 5.

Fig. 5. Inhibition of MMP-9 expression failed to reduce invasion by BB aqueous extracts treatment in NSCLC cells. (A) Western blotting for MMP-9 expression in MMP-9 knockdown cells. MMP-9 knockdown was performed with the shRNA system. Cells (5 × 10⁵) were transfected with the shRNA using viafect reagent according to the manufacturer's instructions. (B) Transfected cells invasion was measured using matrigel coated in 24-transwell chamber plates. BB aqueous extracts containing in the upper chamber and cells (2 × 10⁵) were incubated for 48 h. Invaded cells were visualized by SRB staining and eluted by 10 mM Tris-HCl buffer. Original magnification (200×). Values are expressed as the mean ± SD (**p < 0.01 as compared with the Scramble; ^††p < 0.01 as compared with the BB aqueous extracts -treated group).

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