Fig. 3. p38 activation is required for BB aqueous extracts -induced NSCLC cell apoptosis. (A and B) Cells (1 × 106) were pretreated with SB203580 (20 μM), followed by BB aqueous extracts (100 μg protein/ml) treatment for 24 h. Protein samples were subjected to western blot analysis with antibodies against p-p38, p38, cleaved caspase 3, cleaved PARP, and β-actin. Cell death data quantified by the WST-8 assay are presented as bar graphs. Values are expressed as the mean ± SD (*p < 0.05 as compared with the PBS treated group; †p < 0.05 as compared with the BB aqueous extracts -treated group).