Fig. 6.

Fig. 6. The F1 fraction activates the multicaspase enzyme in MDA-MB-231 cells. (a) Flow cytometry plot for cell viability and multicaspase enzyme analysis of cells treated with the F1 fraction for 48 h. Control group (A), 30 μg/ml group (B), and 60 μg/ml group (C), (b) The bar charts represent the percentages of apoptotic cells post treatment with 30 and 60 μg/ml of propolis extract for 48 h. Values are mean ± SD from three independent experiments (n = 3). **Indicates significantly different values, p < 0.05. live cells (caspase (-) and dead cell marker (7-AAD) (-)); Caspase+: (caspase (+) and dead cell marker (-)); caspase+/Dead: (caspase (+) and dead cell marker (+)); Dead: cells died through necrosis but not via apoptosis (caspase (-) and dead cell marker (+)).

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