Fig. 5.

Fig. 5. F1 fraction-activated apoptosis in MDA-MB-231 cells. (a) Flow cytometry plot for Annexin-V & dead cell analysis of cells treated with different concentrations of the F1 fraction for 24 h. Control group (A), 30 μg/ml group (B), and 60 μg/ml group (C). (b) Percentage of apoptotic cells post treatment with 30, and 60 μg/ml of F1 fraction for 48 h. Values were mean ± SD from three independent experiments (n = 3). **Indicates significant difference between means and differences were considered significant at p < 0.05. VC: vehicle control. L: live cells; EA: early apoptotic cells, LA: late apoptotic cells (cells in late stage of apoptosis or died by apoptotic pathway, AnnexinV-PE(+) and dead cell marker (7-AAD) (+) ); D: dead cells (cells are dead due to necrosis but not via apoptotic pathway, AnnexinV-PE(-) and dead cell marker (+)).

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