Fig. 4.

Fig. 4. Cell morphology, propidium iodide staining, and Caspase 3/7 assay of human MDAMB231 breast cancer cells treated with 30 μg/ml of the F1 fraction for 48 h; the light and the fluorescence microscopy images were captured at 200× magnification. In light microscopy, control cells (A) displayed normal cellular morphology, whereas treated cells (A') showed shrinkage and cell detachment. In fluorescence imaging of PI stained control cells (B) and treated cells (B'), the fragmentation of chromatin (abnormal nuclei) and horseshoe-shaped nuclei in the treated cells indicated cell death. Caspase-3/7 activity was more prominent in treated cells (C') than in control cells (C).

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