Fig. 1.
The pattern of protein histidine phosphorylation changes during brown adipocyte differentiation. (A) The expression patterns of protein histidine phosphorylation in HEK293 cells and C2C12 cells. Cell lysates with (+) or without (-) heat treatment at 95°C for 10 min. (B) The expression patterns of protein histidine phosphorylation during brown adipocyte differentiation without heat treatment. Whole-cell lysates were harvested at the indicated days of differentiation, and analyzed with pHis antibody. Western blot showing the pHis pattern (left), and Ponceau S staining of the membrane shown the protein patterns (right). HSP90 was used as a loading control.