JMB

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Table. 1.

Table. 1.

Bacterial strains and plasmids used in this study.

Strain/plasmid Relevant characteristics Source/reference
Plasmid
pATTP attP-intΦC31-containing pGEM-T easy vector This work
pSBAC accIII(IV), oriT, attP-int, backbone of pCC1BAC [10]
pSA Modified pSBAC which deleted attP-int [11]
pSAMD Modified pSBAC with deleted attP-int and inserted dptPfragment and NheI site This work
pDPT pSAMD with 70kb DNA insert containing whole dpt gene cluster This work
pDPT001 pDPT with attP-intΦC31 This work
E. coli
EPI300 F-mrc-A-D (mrr-hsdRMS-mcrBC) trfA host for cloning and amplification of various BAC vectors and constructs derived from it Epicenter
S17-1 E. colihost for transferring various plasmids into Streptomycesvia conjugation
ET12567/pUZ8002 E. colihost for transferring various plasmids into Streptomycesvia conjugation
Streptomyces roseosporus
ATCC31568 Original daptomycin-producing strain [18]
Streptomyces coelicolor
M511 ΔactII-orf4-deleted M145, Non daptomycin-producing strain [20]
DPT101 M511 with pDPT001 This work
J. Microbiol. Biotechnol. 2019;29:1931~1937 https://doi.org/10.4014.jmb.1909.09022
© J. Microbiol. Biotechnol.