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Fig. 1.

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Fig. 1. Effect of loliolide on HDP spheroid viability. (A) Chemical structure of loliolide drawn by ACD/Chemsketch. (B) Cells were treated with loliolide at the indicated doses for 48 h. Cell viability was determined using a WST-1 assay. (C) Cells were treated with 10, 20, and 50 μg/ml of loliolide for 48 h, and phase-contrast images of spheroids were captured. (D) After treating with loliolide for 48 h, the mRNA expression of growth factors in HDP spheroids was analyzed by qRT-PCR. GAPDH served as an endogenous control. (E) After treating with loliolide for 48 h, the protein expression of growth factors in HDP spheroids was determined using immunoblotting assays with specific antibodies. β-actin served as a loading control. Quantification of the protein levels was performed using ImageJ. The data represent the means of three independent samples ± SD. *p < 0.05 and **p < 0.005 versus DMSO-treated control. Scale bars: C, 200 μm.
J. Microbiol. Biotechnol. 2019;29:1830~1840 https://doi.org/10.4014/jmb.1908.08018
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