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Fig. 4.

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Fig. 4. EP1 mediates PGE2-induced upregulation of Bak. (A) Colonocytes were exposed to DMSO, 10 μM PGE2, 10 μM 11- deoxy PGE1 (EP2/4 agonist), 10 μM butaprost (EP2 agonist), 5 μM 17- phenyl trinor PGE2 (EP1 agonist), or 10 μM sulprostone (EP1/3 agonist) for 2 h. Cell lysates were resolved on 10% polyacrylamide gels and probed with the indicated antibodies. Results shown are representative of three independent experiments (B). Colonocytes were incubated with DMSO, PGE2 (10 μM), or toxin A (3 nM) plus either PLC inhibitor (U-73122, 10 μM) or PKC inhibitor (GF109203X, 10 μM) for 2 h. Cell lysates were resolved on 10% polyacrylamide gels and probed with the indicated antibodies. Results are representative of three separate experiments.
J. Microbiol. Biotechnol. 2019;29:1675~1681 https://doi.org/10.4014/jmb.1906.06040
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